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KMID : 0861020190340010091
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Korea Journal of Herbology
2019 Volume.34 No. 1 p.91 ~ p.98
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Anti-inflammatory effects of Parthenocissus tricuspidata extracts
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Shin Kyung-Soon
Yoo Ji-Hyun
Kil Ki-Jung
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Abstract
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Objectives : While inducing inflammatory response due to LPS it will investigate mechanism associated with anti-inflammatory effects from macrophages and provide basic data for the possible use as anti-inflammatory materials.
Methods : We investigated cell viability, NO, TNF- and IL-6 by ELISA and expressions of iNOS, COX-2, MAPKs and NF-B were measured in RAW 264.7 cells induced by LPS.
Results : The cell viability of Parthenocissus tricuspidata extracts(PTE) identified in macrophages showed that cell viability rate was more than 99% at the concentration of 8, 40, and 200 g/mL. NO generated amounts revealed that it relied on concentration and was significantly reduced compared to the control. The expression of iNOS was restrained by the control at the concentration of 200 and 400 g/mL. In addition, the expression of COX-2 was found to be significantly reduced to the untreated control at the concentration of 400 g/mL. TNF- relied on concentration and showed a significant decreased compared to the control. In contrast, IL-6 relied on concentration, reduced compared to the control. Phosphorylation of ERK, JNK, and p38 mediated by LPS were restrained by relying on concentration. Phosphorylation and decomposition of IB as well as p65 nuclear transmission of NF-B subunit were restrained.
Conclusions : By restraining the activation of NF-B, anti-inflammatory effects were revealed by reducing phosphorylative activation of MAPKs, restraining the expression of iNOS and COX-2 and restraining the creation of NO, IL-6, and TNF-. Therefore, it can be assumed that they can be used as a variety of anti-inflammatory materials.
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KEYWORD
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Parthenocissus tricuspidata, Anti-inflammatory, NF-B, Macrophage cell, LPS
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